In March or in early April (likely 4 April) 1945, she had a litter of five puppies with Gerdy Troost's German Shepherd, Harras. Adolf Hitler named one of the puppies "Wulf", his favorite nickname and the meaning of his own first name, Adolf ("noble wolf"), and he began to train her. One of Blondi's puppies was reserved for Eva Braun's sister Gretl. Eva sent Gretl a letter containing a photo of Blondi and three of her puppies, Gretl's being indicated with an arrow.

According to a report commissioned by Joseph Stalin and based on eyewitness accounts, Hitler's dog-handler, Feldwebel Fritz Tornow, took Blondi's pups and shot them in the garden of the bunker complex on 30 April 1945. After the battle in Berlin ended on 2 May 1945, the remains of Hitler, Braun, and two dogs (thought to be Blondi and her offspring Wulf) were discovered in a shell crater by a unit of SMERSH, the Soviet counter-intelligence agency. The dog thought to be Blondi was exhumed and photographed by the Soviets.